This simple datapath is of a single-cycle nature. The instruction begins with the PC.
SUB Instruction

The SUB instruction performs a subtraction on the first source register's contents by the second source
register's
contents, and stores the result in the destination register.

It's syntax is:
SUB $destination register's address, $first source register's address, $second source register's address.

The sample SUB instruction demonstrated in the datapath above is SUB $8, $2, $6.

The instruction's equivalent in binary is:

Information on the content of the registers used:
Register's Address Register's Address (Binary) Register's Value Register's Value (Binary)
$2 00010
$6 00110
$8 01000 To be updated To be updated