Research


secA in protein translation secA in protein translation

Role of SecA in protein translocation

We are interested in determining 3D structures of macromolecular protein complexes to understand their function and mechanism. We employ Cryo-Electron Microscopy with single particle reconstruction as our main tool for structural determination. Our laboratory is equipped with advanced Electron Microscopes and high speed computing clusters for routine single particle reconstruction work.

We are mainly interested in the following research areas:

  • Protein translocation across cellular membranes
  • Co-translational protein folding
  • Protein secretion
  • Translational stalling and regulation

 

mitochondrial chaperonine 1 mitochondrial chaperonine 2

mitochondrial chaperonine 3

Mitochondrial Chaperonine (neg-stained)

single particle reconstruction

Cryo-EM and single particle reconstruction

monomeric and dimeric secA bound to ribosome

Structures of a monomeric- and dimeric- SecA bound to the ribosome (10 and 8.5 Å)